The pedagogical competencies of instructor are related to methods, attitudes and procedures necessary to the teachinglearning process. Motivate learning, use a clear and friendly language, accept the heterogeneity of knowledge, stimulate critical capacity, evaluate the development and provide feedbacks of assignments are skills required in e-learning. Perceptions about pedagogical competencies of ten instructors of undergraduation or graduation degrees of Rio de Janeiro State (Brazil) institutions were investigated by a questionnaire. Subjects had pedagogical competencies as vital to instruction. Its improvement occurred with professional development, especially via short-term on-line courses. However, few could maintain a continuous degree. The most important pedagogical competence would be the ability to develop the critical skills of the students. However, instructors pointed out a better performance in motivate learning, get student empathy, use clear and friendly language and provide feedbacks of assignments. Interestingly, the most important competencies have been identified as more difficult to develop. Subjects indicated assessing learning correctly as the more difficult competence to apply. One reason would be the need to employ objective correction criteria but, at the same time, consider the subjectivity of the student, in other words, the individual development of each one